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2014 No.1 Jan - Mar - Research

Pengaruh Autoclave terhadap Properti Fisik dan Histopatologi Tulang: Sebuah Penelitian Dasar Rekonstruksi

I GEDE EKA WIRATNAYA1, ACHMAD FAUZI KAMAL1, RINO PATTIATA2, EVELINA KODRAT2

1Department of Orthopaedic and Traumatology, Cipto Mangunkusumo Hospital Faculty of Medicine Universitas Indonesia
2Department of Patology AnatomyCipto Mangunkusumo Hospital Faculty of Medicine Universitas Indonesia

Diterima 19 Februari 2014; Direview 18 Maret 2014; Disetujui 27 Maret 2014

ABSTRACT

Background: Autoclave autograft is a promising alternative procedure in surgical treatment of bone tumors. A minimum effective autoclaving time of 15 minutes at 134°C to devitalise tumour cells. This study aims to know the effect of autoclave to physical properties and histopathology of bone.

Methods: We performed experimental study in 28 femoral bone of goat (Capra Aergagus circus) at age 1?2 year old. We divide into two groups with simple random sampling to be 14 autoclave and 14 control group. Autoclaving was performed to the bone at 134°C for 15 minute. We examined physical properties each 7 autoclave group to axial and bending force compared with physical properties of the control group. We also examined histopatology of the bone both groups. Independent t-test statistical analysis to evaluate the difference of axial and bending force between autoclave and control group.

Results: There were significant difference between autoclave and control group in axial force (p = 0.000) and bending force (p = 0.03). Physical properties of autoclave bone decreased 58.58 % in axial force and 20.33% bending force to control group. Histopathology examination revealed that all osteocytes were death in autoclave group but without matrix changes.

Conclusion: Autoclave at 134°C for 15 minute caused physical properties of bone decrease and statistically significant difference. It also made all osteocytes died but without matrix disturbance.

Keyword: Autoclave bone, Physical properties of bone.

ABSTRAK

Autograft yang mendapat pajanan autoclave merupakan salah satu prosedur alternatif dalam penatalaksanaan bedah tumor tulang. Pemanasan autoclave efektif untuk devitalisasi sel tumor pada suhu 134°C dalam waktu minimal 15 menit. Studi ini bertujuan untuk mengetahui pengaruh autoclave terhadap properti fisik dan histopatologi tulang.

Penelitian ini merupakan studi eksperimental terhadap 28 tulang kambing (Capra Aergagus circus) usia 1?2 tahun. Kami membagi menjadi dua kelompok dengan simple random sampling menjadi 14 kelompok autoclave dan 14 kelompok kontrol. Autoclave dilakukan untuk tulang pada suhu 134°C selama 15 menit. Evaluasi properti fisik dilakukan terhadap masing-masing kelompok 7 sampel tulang untuk gaya axial dan gaya bending. Kami juga memeriksa histopatologi tulang kedua kelompok. Analisis t-test independent statistik untuk mengevaluasi perbedaan axial dan gaya bending antara autoclave dan kelompok kontrol.

Hasil penelitian menunjukkan terdapat perbedaan yang bermakna antara kelompok autoclave dengan kelompok kontrol pada uji gaya axial (p = 0,000) dan gaya bending (p = 0,03). Properti fisik tulang diautoclave menurun 58,58% pada gaya axial dan 20,33% pada gaya bending. Pemeriksaan histopatologi menunjukkan kematian osteosit pada kelompok autoclave.

Penelitian ini menyimpulkan bahwa autoclave mengurangi kekuatan mekanik tulang, baik terhadap gaya axial maupun gaya bending dan bermakna secara statistik, serta menyebabkan kematian seluruh osteosit.

Kata Kunci: tulang autoclave, properti fisik tulang.

 

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